The major albumin proteins from pea (Pisum sativum L). Purification and some properties
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Purification and Characterization of Ornithine Transcarbamylase from Pea (Pisum sativum
Pea (Pisum sativum) omithine transcarbamylase (OTC) was purified to homogeneity from leaf homogenates in a single-step procedure, using 5-N-(phosphonacetyl)-L-omithine-Sepharose 6B affinity chromatography. The 1581-fold purified OTC enzyme exhibited a specific activity of 139 micromoles citrulline per minute per milligram of protein at 370C, pH 8.5. Pea OTC represents approximately 0.05% of the...
متن کاملProperties of an Aminotransferase of Pea (Pisum sativum L.).
A transaminase (aminotransferase, EC 2.6.1) fraction was partially purified from shoot tips of pea (Pisum sativum L. cv. Alaska) seedlings. With alpha-ketoglutarate as co-substrate, the enzyme transaminated the following aromatic amino acids: d,l-tryptophan, d,l-tyrosine, and d,l-phenylalanine, as well as the following aliphatic amino acids: d,l-alanine, d,l-methionine, and d,l-leucine. Of othe...
متن کاملSome properties of spectrin-like proteins from Pisum sativum.
Proteins cross-reacting with antibodies directed against alpha- and beta-spectrin were recently detected in plant cells. In this report we have studied the ability of these proteins to interact with other components of membrane skeleton such as ankyrin, f-actin and calmodulin. It was found that the polypeptide of high molecular weight reacting with anti-alpha-spectrin antibody binds calmodulin ...
متن کاملPea (Pisum sativum L.) Growth Mutants
This review is devoted to the Pisum sativum L. growth mutants. These mutants are presented by several groups of plants. The first group is characterized by short internodes (dwarf mutants le, na, lk, la, lh, ls) or by elongated internodes (slender mutant sln). The stem growth of mutants of both types (dwarf and slender) is controlled by hormones (GA and IAA) in the stem or in seeds. Mutants cha...
متن کاملPlasma Membrane Protein from Etiolated Pea (Pisum sativum L.) Seedlings1
When crude microsomal membranes from apical stem segments of etiolated Pisum sativum L. cv Alaska are mixed in vitro with 'y-[32P]ATP, a phosphorylated band of apparent molecular mass 120 kilodaltons can be detected on autoradiographs of sodium dodecyl sulfate electrophoresis gels. If the stem sections are exposed to blue light immediately prior to membrane isolation, this band is not evident. ...
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ژورنال
عنوان ژورنال: Biochemical Journal
سال: 1984
ISSN: 0264-6021,1470-8728
DOI: 10.1042/bj2180795